1. Perform procedure in triplicate with blank trial run concurrently with samples.

2. Weigh a minimum of 0.05±0.005 g of sample into a glass vial. ( Collect more sample if it is not uniform to reduce sample variance. ).

3. Add 5 ml of TetraHydroFuran to the sample. ( For samples larger than 0.05 g, add 10 ml of THF for every 0.1 g of sample or a reasonable amount to dissolve sample).

4. Shake sample for at least 30 minutes to completely dissolve.

( Some materials may not dissolve completely. In this case, add an additional two hours to shaking time and then proceed .)

5. Add 10 ml of hexane for every 5 ml of TetraHydroFuran used in previous step to precipitate any PVC polymer.

6. Allow at least 5 minutes for polymer to settle (longer times may be necessary to minimize clogging of filters in subsequent step).

7. Filter THF/hexane solution through a 0.45 ?m PTFE filter.

8. Collect at least 2 ml of filtered solution

9. Combine 0.1 ml* of the filtered THF/hexane solution with 80 ?l of internal standard (BB, 250 ?g/ml) and dilute to 20 ml with cyclohexane.

( A volume of 1.0 THF/hexane may also be used to produce results in the calibration range. )

7. Transfer diluted sample to a GC-MS ready vial for analysis.

8. 1 uL of concentrated extract was injected into GC-MS.

9. The identification of phthalates are performed by comparing the GC retention tmes and mass spectra of phthalate standards.

10. From the results, concentrations of the phthalates present in the respective samples are calculated using linear regression.


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